Tsingke china

Web(Tsingke, China). All primers were designed and synthesized by Tsingke (Guangzhou, China). The expression of small nuclear RNA U6 or β-actin genes was used as a control to calibrate the origi-nal concentration of miRNA, circRNA … Webgel recovery kit (Tsingke, China), ligated with the pClone007 vector (Tsingke, China), and transformed into competent Escherichia coli cells. After transformation, six positive clones were picked for sequencing (Tsingke, China). To obtain the promoter sequence, a PCR gene walking approach was performed by using a universal genomewalk™

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WebJul 7, 2024 · The purified product was used as template for PCR amplification. PCR was performed by using 2× TSINGKE Master mix (TSINGKE, Beijing, China) according to the … WebAll amplification primers were synthesized by Tsingke (China, Beijing). We use a one-step cloning kit (C113, Vazyme, China) to assemble recombinant plasmids (constructions), including Reporter 01-03; Controller 01-06; Switch 01-06; Effector 01-03 (full HlpAmstrain information is provided in Supplementary Materials). citi forward credit cards https://thepegboard.net

PD-L1 lncRNA splice isoform promotes lung ... - Genome Biology

WebChinese G8 strains were obtained in each segment to determine whether the two strains had reassortment between the segments. Results Genotypes and genetic characteristics of Chinese G8 rotavirus Sixteen samples collected from hospitalized AGE children were tested for rotavirus antigen by a commercial EIA kit, resulted in 5 positives. WebJul 15, 2024 · The PCR assay was carried out in a 50 microliter (µL) reaction volume containing 25 µL Extaq Mix (TSINGKE, China), 10 pmol each of the forward and reverse primers (Supplementary Table 1), and 10 ng of template DNA. The PCR cycling conditions were as listed in Sect. 2.2 above. WebSep 23, 2024 · Five or more clones of per amplicon were fully sequenced (TsingKe, China). The final viral genomic sequences were assembled and submitted to NCBI with GenBank accession numbers MH137740.1 (RNA1) and MH137741.1 (RNA2). By HTS analyses, the average coverage of reads mapped on the RNA1 and RNA2 is 1934 and 3640, ... citi forward for college students review

Genetic diversity analysis of Chinese Leishmania isolates and ...

Category:Tobacco leaf curl Puer virus: a novel monopartite ... - Springer

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Tsingke china

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WebDescription. Gene Synthesis is a reliable and cost-effective method for obtaining customized DNA constructs with 100% sequence accuracy. As a powerful molecular biology tool, DNA … WebDescription. Gene Synthesis is a reliable and cost-effective method for obtaining customized DNA constructs with 100% sequence accuracy. As a powerful molecular biology tool, DNA synthesis is rapidly becoming a crucial part of research workflow and has transformed modern biology. De novo gene synthesis is quickly replacing the classical cloning ...

Tsingke china

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WebMar 10, 2024 · China is one of the main sugar producers in the world while Guangxi is the largest sugarcane and sugar-producing area in ... Germany) by using TSINGKE Biological Technology (www.tsingke.net) PCR primers . The PCR mixture ingredients were 2 × TSINGKE (China) master mixes (blue), 2× (10 μM) forward, reverse primers ... WebApr 13, 2024 · The labeled probes with biotin (TSINGKE Biological Technology, China) and labeled RNAs were incubated with A549 cell nuclear extracts; then, the RNA pull-down assay was performed using the Pierce Magnetic RNA Protein Pull-Down Kit (20164, Thermo Fisher Scientific, USA). The proteins were analyzed by western blotting.

WebDec 18, 2024 · Biotinylated circARHGAP12 probe (Tsingke, Wuhan, China) was cultivated with streptavidin magnetic beads (Invitrogen) at room temperature for 2 hours to produce … WebMay 5, 2024 · Tsingke Biotechnology General Information. Description. Developer of synthetic genomics and biosynthetic products. The company's business covers three …

WebJun 9, 2024 · Rapid gene synthesis of ultra long fragments is one of the most difficult technologies in the field of gene synthesis. As a public service platform for large segment gene synthesis and precise sequencing, recently, the ultra long segment rapid gene synthesis technology developed by Tsingke biology has successfully broken through the … WebJun 9, 2024 · Rapid gene synthesis of ultra long fragments is one of the most difficult technologies in the field of gene synthesis. As a public service platform for large segment …

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WebJun 9, 2024 · Reward Measures for Publishing Scientific and Technological Papers of Tsingke Biology in 2024 . In order to thank our customers for their support and trust in … diary\\u0027s o2WebMay 21, 2024 · RAPD amplification was performed in 50 μl reactions containing 0.8 μM primer, 10 ng of genomic DNA sample, 25 μl of 2 × TaqMater Mix (Tsingke, China) and PCR-grade distilled water. The PCR procedure was as follows: initial denaturation at 94 °C for 5 min followed by 45 cycles of 94 °C for 1 min, 36 °C for 1 min, 72 °C for 2 min, and a final … citi forward card reviewciti foundation careersWebBased in Beijing, China, and established in 2024, Tsingke Biotechnology Co., Ltd. is a biotechnology company that focuses on R&D of synthetic genomics and biosynthetic … citi foundation addressWebApr 10, 2024 · The ZDHHC3 lentiviral short hairpin RNA (shRNA) vector was designed and purchased from Tsingke (Beijing, China) and was used to construct stable transfection Panc 02 pancreatic cell lines. The PD-L1 overexpressing in ZDHHC3-knockdown Panc 02 cell line was constructed by a recombinant lentivirus (pLV-PD-L1-puro) and performed following … diary\u0027s o4WebApr 9, 2024 · The procedures were performed according to the user manual provided with the kit. All PCR products were purified using a FastPure Gel DNA Extraction Kit (Vazyme, China), cloned into the pMD TM 18-T vector, and then introduced into chemically competent E. coli JM109 cells (TSINGKE, China) for propagation. diary\u0027s o2WebJan 30, 2024 · The following is the second piece in this series, conducted by our analyst Mianmian Wang after her talk with Dr.Du, Vice President of Tsingke Biotechnology (Chinese: 擎科生物). Tsingke Biotechnology is a gene synthesis platform enterprise, equipped with the whole industry chain, covering the business scope of synthetic genomics products and ... citi foundation florencia