Web(Tsingke, China). All primers were designed and synthesized by Tsingke (Guangzhou, China). The expression of small nuclear RNA U6 or β-actin genes was used as a control to calibrate the origi-nal concentration of miRNA, circRNA … Webgel recovery kit (Tsingke, China), ligated with the pClone007 vector (Tsingke, China), and transformed into competent Escherichia coli cells. After transformation, six positive clones were picked for sequencing (Tsingke, China). To obtain the promoter sequence, a PCR gene walking approach was performed by using a universal genomewalk™
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WebJul 7, 2024 · The purified product was used as template for PCR amplification. PCR was performed by using 2× TSINGKE Master mix (TSINGKE, Beijing, China) according to the … WebAll amplification primers were synthesized by Tsingke (China, Beijing). We use a one-step cloning kit (C113, Vazyme, China) to assemble recombinant plasmids (constructions), including Reporter 01-03; Controller 01-06; Switch 01-06; Effector 01-03 (full HlpAmstrain information is provided in Supplementary Materials). citi forward credit cards
PD-L1 lncRNA splice isoform promotes lung ... - Genome Biology
WebChinese G8 strains were obtained in each segment to determine whether the two strains had reassortment between the segments. Results Genotypes and genetic characteristics of Chinese G8 rotavirus Sixteen samples collected from hospitalized AGE children were tested for rotavirus antigen by a commercial EIA kit, resulted in 5 positives. WebJul 15, 2024 · The PCR assay was carried out in a 50 microliter (µL) reaction volume containing 25 µL Extaq Mix (TSINGKE, China), 10 pmol each of the forward and reverse primers (Supplementary Table 1), and 10 ng of template DNA. The PCR cycling conditions were as listed in Sect. 2.2 above. WebSep 23, 2024 · Five or more clones of per amplicon were fully sequenced (TsingKe, China). The final viral genomic sequences were assembled and submitted to NCBI with GenBank accession numbers MH137740.1 (RNA1) and MH137741.1 (RNA2). By HTS analyses, the average coverage of reads mapped on the RNA1 and RNA2 is 1934 and 3640, ... citi forward for college students review