Psin4-ef2-ires-pur
WebJan 7, 2024 · pSIN4-EF2-IRES-Pur backbone_mutation backbone_origin backbone_size 5500 promoter sequencing_primer_3 sequencing_primer_5 vector_types Mammalian Expression Lentiviral Other reprogramming depositor comments : After lentivirus infection, Klf4 and cMyc are coexpressed by CMV promoter and IRES intron. WebG@ Bð% Áÿ ÿ ü€ H FFmpeg Service01w ...
Psin4-ef2-ires-pur
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WebAug 7, 2009 · Supernatants derived from pSIN4-EF2-IRES-Neo were used to transduce sibling H9 cells as a mock control. Forty-eight hours after transduction, 300 μg/ml neomycin was added daily for selection of cell clones expressing the transgenes. Stable clones expressing ABCG2 or the mock control were obtained 12-15 days after transduction and … WebFeb 17, 2010 · Abstract. Derivation of induced pluripotent stem (iPS) cells requires the expression of defined transcription factors (among Oct3/4, Sox2, Klf4, c-Myc, Nanog, a
WebIP67. Operational voltage. 24V DC ± 5％ (incl. ripple) Product Notes: Please check the product specification for the part number RPE-1804N-PU-02 on the OUTPUT SENSOR RPE … WebIn the present study, we indicated that the expression of miR-203 was significantly downregulated in serum samples derived from patients with cerebral infarction and in mice brain samples following...
WebImaged and quantified results revealed that when PC12 cells and primary neurons, infected with Ad-GFP-LC3, were exposed to Cd (10 and 20 μM) for 8–24 h, the ratio of the cells with large LC3 puncta structures significantly increased compared to that of the vehicle-treated cells ( Fig. 1C and D ). Fig. 1. WebJan 7, 2024 · pSIN4-EF2-IRES-Pur backbone_mutation backbone_origin backbone_size 6300 promoter sequencing_primer_3 sequencing_primer_5 vector_types Lentiviral Other reprogramming depositor comments : After lentivirus infection, Oct4 and Sox2 are coexpressed by EF2 promoter and IRES intron.
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WebLentiviral Backbone Plasmid Psin4 Ef2 Ires Pur, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … good root of title unregistered landWebpSin4-EF2-IRES-Pur (Search Vector Database) Backbone size w/o insert (bp) 6777 Vector type Mammalian Expression, Lentiviral, Cre/Lox Growth in Bacteria Bacterial Resistance … chest of drawers for sale farmington moWebFeb 28, 2024 · The PCR products of FLAG-Parkin, FLAG-ATG5, and EGFP were cloned into pSin4-EF2-IRES-Pur vector via EcoRI/BamHI double-digestion. The above primers used are listed in Table 1. To generate lentivirus, the above constructed plasmids were co-transfected together with pMD2.G and psPAX2 (Addgene, Cambridge, MA, USA) to 293TD cells using … chest of drawers for baby roomWebreplacing EGFP in the pSIN4-EF2-EGFP-IRES-Neo plasmid (courtesy of Dr. James Thomson), resulting in pSIN4-EF2-ABCG2-IRES-Neo. Alternatively, EGFP cassette was deleted by BamHI to generate pSIN4-EF2-IRES-Neo as a negative control. Each of the two plasmids was used to prepare lentivirus in 293FT cell line (Invitrogen) with Superfect (Qiagen ... chest of drawers for sale gold coast gumtreeWebTo optimize reprogramming of fibroblasts from patients with A-T, we examined the response of A-T cells to various cell culture conditions after lentiviral transduction with reprogramming factors Oc4/Sox2 (pSIN4-EF2-O2S) and Klf4/c-Myc (pSIN4-CMV-K2M). good rooms for boysWebÿþÿ SketchUp Modelÿþÿ {18.0.16975}.nz XrC³5MXcmÔhÿþÿ¿‚œ`ÿÿ CVersionMapÿþÿ CArcCurve ÿþÿ CAttributeÿþÿ CAttributeContainerÿþÿ CAttributeNamed ÿþÿ CBackgroundImage ÿþÿ CCamera ÿþÿ CComponent ÿþÿ CComponentBehavior ÿþÿ CComponentDefinition ÿþÿ CComponentInstance ÿþÿ CConstructionGeometryÿþÿ … good roots coffeeWebApr 1, 2024 · The PCR products of FLAG-XIAP and EGFP were cloned into pSin4-EF2-IRES-Pur vector via EcoRI/BamHI double-digestion. To package lentivirus, the constructed plasmids were co-transfected together with pMD2.G and psPAX2 (Addgene, Cambridge, MA, USA) to 293TD cells using MegaTran 1.0 reagent (OriGene Technologies, Rockville, MD, … chest of drawers for sale in dothan al