WebThe firefly LCI transient expression assay was performed as described previously . N. benthamiana leaves were coinfiltrated with A. tumefaciens strains carrying the binary plasmids of pCAMBIA1300:COP1-nLUC and pCAMBIA1300-cLUC-PID. The corresponding empty vector pCAMBIA1300-nLUC or pCAMBIA1300-cLUC was infiltrated as a negative … WebNov 9, 2011 · First, our assay adopted different firefly luciferase fragments in complementation, the combination of which has been shown to generate higher signal-to …
Firefly Luciferase Complementation Imaging Assay
WebConstructs for LCI Assays The firefly LUC fragments 2-416 (NLuc) and 398-550 (CLuc) were successfully used for protein-protein interaction assays in the mammalian system (Luker et al., 2004). These two fragments roughly correspond to the independently folded N-terminal and C-terminal domains that are linked by a disordered flexible region WebLuciferase assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of luciferase assay products and tools for your research. Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes ... buddhist healing mantra
Luciferase Assays Luciferase - Promega
WebD, Firefly LCI assay confirming interaction between OsSCD2 and OsSCD1 in N. benthamiana leaves. nLUC, cLUC, and OsCNGC9 were used as controls. Colored scale bar indicates the luminescence ... WebDec 1, 2024 · The yeast two-hybrid system, firefly luciferase complementation imaging (LCI) assay and bimolecular fluorescence complementation (BiFC) assay further confirmed that BTB proteins interact with several other proteins to perform a certain developmental process in plants. ... [31] was used for the investigation of protein interaction of OsBTB70 ... WebAug 10, 2024 · Firefly LCI Assays. LCI assays were performed as described previously (Chen et al., 2008). The LUC n - and LUC c-fused plasmids were transformed into Agrobacterium strain GV3101, and the indicated transformants pairs were infiltrated into N. benthamiana leaves. The plants were grown in the light for 3 d, and luciferase activity … buddhist head